Research

Research

How does C60 work as an anti-inflammatory antioxidant?

The C60 Buckyball molecule is an electron reservoir that neutralises free radicals. Free radicals are molecules that have one or more electrons too few or too many, and they will damage any biological molecule they come in contact with. The Buckminster-fullerene C60 molecule can accept several electrons in case of a free radical with an electron surplus, and donate several electrons in case of a free radical with an electron deficit. C60 thus is an incredibly effective antioxidant, not in the least because it does not get degraded or become unstable by accepting or donating electrons.

The olive oil of the lipofullerene C60 binds the C60 into the cell layers, also those of the mitochondria, the energy factories in the cell. Hence the cells and mitochondria get an integrated nano-scale antioxidant system implanted into their cell walls. This is totally unique. No other antioxidant supplement can be reused indefinitely. No other antioxidant supplement can both accept and donate electrons, let alone multiple electrons. No other antioxidant supplement sits for weeks in the lipid bilayers, exactly where it is needed the most – except for C60, which can do all these things.

One way C60 functions as an antioxidant, is to neutralize Reactive Oxygen Species (ROS) by accepting an electron from them. The unique aspect of C60 is that accepting an electron does not alter the structure of the C60 molecule, effectively infinitely recycling it. C60 can accept or donate many electrons (it works as an electron reservoir), so it is an incredibly powerful antioxidant in the cell walls (270 x better than vit. C, even disregarding the recycling effect!), constantly neutralizing damaging free radicals that reach the cells or mitochondria.

Countless studies have shown that C60 fullerene could have many potential biomedical applications. Several independent research groups showed that C60 has no acute or sub-acute toxicity in various experimental models. Oral administration of C60 dissolved in olive oil (0.8 mg/ml) at repeated doses (1.7 mg/kg of body weight) to rats not only Does Not cause chronic toxicity but it almost Doubles Their Natural Lifespan.

The effects of C60-olive oil solutions in an experimental model of CCl4 intoxication in rat strongly suggest that the effect on lifespan is mainly due to the reduction of age-associated increases in oxidative stress.

Pharmacokinetic studies show that dissolved C60 is absorbed by the gastro-intestinal tract and eliminated in 20 to 30 hours. Carbon 60 fullerene can distribute more or less evenly in the organism and penetrate in various tissues and organs. It is also well absorbed through the skin.

These results of importance in the fields of medicine and toxicology should open the way for the many possible – and waited for – biomedical applications of C60 including as an adjunct in cancer therapy, neurodegenerative disorders, and ageing.

Carbon 60 (C60 – Lipofullerene) is Totally Non-Toxic and has in fact been shown to have “Negative Toxicity”.  Its use has demonstrated stronger growth and longer life in several trials on lower as well as higher organisms.

The original study by done by Tarek Baati and his research team in 2011, showed that Carbon 60 almost doubled the normal lifespan of rats. When the last rat in the control group had died, all rats in the C60-olive oil group were still alive and well – they went on to live for an average of two more years!

 

The original study that inspired global excitement about the use of Carbon 60 Olive Oil:

The Prolongation of the Lifespan of Rats by Repeated Oral Administration of [60]Fullerene

(also known as The University of Paris Study or The Baati Study)
Biomaterials, Volume 33, Issue 19, June 2012, Pages 4936-4946. Available Online 10 April 2012
Tarek Baati, Fanchon Bourasset, Najla Gharbi, Leila Njim, Manef Abderrabba, Abdelhamid Kerkeni, Henri Szwarc, and Fathi Moussa

 “Abstract: Countless studies showed that [60]fullerene (C60) and derivatives could have many potential biomedical applications. However, while several independent research groups showed that C60 has no acute or sub-acute toxicity in various experimental models, more than 25 years after its discovery the in vivo fate and the chronic effects of this fullerene remain unknown. If the potential of C60 and derivatives in the biomedical field have to be fulfilled these issues must be addressed. Here we show that oral administration of C60 dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) to rats not only does not entail chronic toxicity but it almost doubles their lifespan. The effects of C60-olive oil solutions in an experimental model of CCl4 intoxication in rat strongly suggest that the effect on lifespan is mainly due to the attenuation of age-associated increases in oxidative stress. Pharmacokinetic studies show that dissolved C60 is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours. These results of importance in the fields of medicine and toxicology should open the way for the many possible -and waited for- biomedical applications of C60 including cancer therapy, neurodegenerative disorders, and ageing.”

Full study available for rent or purchase here: http://www.sciencedirect.com/science/article/pii/S0142961212003237

Charts associated with this study are linked below. Authors of the above paper have issued several corrections, most notably a correction to the survival chart, figure 3. These charts reflect the corrected data: http://www.sciencedirect.com/science/article/pii/S0142961212005522


Another study exploring the antioxidant qualities of Carbon 60:

Possible Mechanisms of Fullerene C60 Antioxidant Action

Biomed Research InternationalPublished online 2013 Oct 8. 
V. A. Chistyakov, Yu. O. Smirnova, E. V. Prazdnova, and A. V. Soldatov 

 “Abstract: Novel mechanism of antioxidant activity of buckminsterfullerene C60 based on protons absorbing and mild uncoupling of mitochondrial respiration and phosphorylation was postulated. In the present study we confirm this hypothesis using computer modeling based on Density Functional Theory. Fullerene’s geroprotective activity is sufficiently higher than those of the most powerful reactive oxygen species scavengers. We propose here that C60 has an ability to acquire positive charge by absorbing inside several protons and this complex could penetrate into mitochondria. Such a process allows for mild uncoupling of respiration and phosphorylation. This, in turn, leads to the decrease in ROS production.”

Full study available here: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816026/

 

Much more research on this amazing product is currently underway!

Research Links:
https://www.sciencedirect.com/science/article/pii/S0142961212003237
https://www.ncbi.nlm.nih.gov/pubmed/22498298
https://www.ncbi.nlm.nih.gov/pubmed/?term=12054749
https://www.ncbi.nlm.nih.gov/pubmed/?term=16351219

 

 

Research